摘要
采用改进的逆相蒸发法（ＲＥＶ）制备卵磷脂／胆固醇（７：３，ｍｏｌ／ｍｏｌ）脂质体，并用于包裹荧光特异标记的质粒ＰＢＲ３２２ＤＮＡ，构建了基因转移载体，然后将纯化的质粒ＰＢＲ３２２ＤＮＡ－脂质体与悬浮的白菜原生质体一起温育．经对ＤＮＡ－ＤＡＰＩ荧光复合物的镜检，证明ＤＮＡ被包裹到卵磷脂／胆固醇脂质体内．被包装的ＤＮＡ能有效地避免外源ＤＮＡａｓｃ的降解作用．且质粒ＤＮＡ通过脂质体与原生质体膜的融合可随脂质体导入原生质体内．
Using improved reverse phase evaporation(REV) method, We prepared EPC/Cholesterol (7: 3, mol/mol) liposomes and encapsulated a fluorecent specific marked plasmid with them. Result in Constructing a gene transfer carrier. Then,Pure DNA-liposomes were incubated with Brassica chinensis protoplasts. We examined the DNA-DAPI fluorescent mixture wraped in liposomes by fluorecent microscope. The encapsulated DNA avoids effectively the degradation of exogenous DNase and the plasmid DNA was conducted into protoplasts through liposome fusion with protoplast membrame.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
1995年第3期372-377,共6页
Journal of Central China Normal University：Natural Sciences
