摘要
以甘蓝型波里马胞质雄性不育系油菜（以下简称ｐｏｌＣＭＳ）为母本，与花椰菜杂交，在授粉后不同天数，利用不同的培养基对其种间杂种的子房进行培养，发现不同培养基的培养效率明显不同。利用Ｗｈｉｔｅ（１９６３）和Ｎｉｔｓｃｈ（１９５６）培养基培养所获得的种子数和幼苗数均多于ＭＳ和１／２ＭＳ培养基。从幼苗数目来看，培养的时间越早，其幼苗数目越多。
Polima cytoplasm male sterlity Brassica nap us was as maternal plant to cross with Brassica oleracea var. botrytis. Ovaries of interspecific hybrid with different days of pol- lination were cultured in different culture media.It showed that the culture effect was varied in different media.More seeds and plantlets were obtained by using White or Nitsch culture medium than MS or a half MS medium. To sum up the number of plantlets ,the earlier cul- ture was practiced the more plantlets were obtained.
出处
《湖北农业科学》
1995年第5期46-49,共4页
Hubei Agricultural Sciences
基金
武汉市科委"晨光计划"项目
关键词
油菜
甘蓝型油菜
花椰菜
子房培养
种间杂交
Brassica nap us
Brassica oleracea var.botrytis
ovary culture
number of seeds
number of plantlets
