摘要
本文采用聚合酶链反应（ＰＣＲ）方法从９例抗－ＨＢｅ阳性的乙肝患者血清中扩增了含ＰｒｅＣ和Ｃ基因的ＤＮＡ片段，并分别与载体ｐＵＣ１８相连接，成功地克隆入大肠杆菌中，为后续的ＤＮＡ测序及分析ＰｒｅＣ基因突变打下了基础。并建立了快速、简便的ＰＣＲ直接筛选和鉴定阳性克隆的方法。
For the purpose of analysing HBV PreC gene mutations,a
specific HBV DNAfragment including PreC and C gene was amplified by PCR from the sera of 9
patients posi-tive for Anti-HBe,and cloned into the vector pUC18.A simple and rapid method for
screen-ing positive clones by PCR was also established in this paper.
出处
《河南医学研究》
CAS
1995年第4期308-310,共3页
Henan Medical Research
