摘要
本文报道了油菜叶绿体16S rRNA基因的全顺序及其5′端上游的156bp和3′端下游的101bp的核苷酸顺序。油菜叶绿体16s rRNA基因长为1491bp,和烟草、玉米相比,同源程度分别为98.5％、96.1％。油菜叶绿体16S rRNA基因5′端上游及3′端下游的顺序能互补而形成一个较大的茎环结构,但与烟草相比,由于3′端下游顺序有79bp的缺失,因此,该结构中的茎部分大小仅为烟草的二分之一。
The complete nucleotide sequence of a 16S rRNA gene and its flanking sequence from Brassica napiis chloroplast has been determined.The 16S rRNA gene is 1491 bp in length,having 98.5%,and 96.1% homology with those of tobacco and maize chloroplast,respectively.The large stem and loop structure can be constructed from the sequence surrounding the 5' and 3' ends of the 16S rRNA gene.The stem is about half shorter than that of tobacco,due to a 179 bp deletion in Brassica napus chloroplast.
