摘要
目的：观察［￣３Ｈ］组胺在心肌细胞粗制膜上的结合情况．方法：制备心肌细胞粗制膜，将膜制备与［￣３Ｈ］组胺在３７℃下共同孵育３０ｍｉｎ，然后将孵育液经玻璃纤维膜减压抽滤．滤膜烘干后放入闪烁瓶中，记录放射活性．结果：选择性Ｈ－３受体激动剂α－甲基组胺（α－ＭｅＨＡ，１μｍｏｌ／Ｌ）可竞争性抑制［￣３Ｈ］组胺在心肌膜制备上的特异性结合，［￣３Ｈ］组胺（０．５和１ ｎｍｏｌ／Ｌ）特异性结合量减少至对照值的６４．１±１１．１％和６０．５１±４．１％。６－羟基多巴胺（６－ＯＨＤＡ）化学切割交感神经后，可以使［￣３Ｈ］组胺与膜受体的结合量减少至对照值的７７．３±６．９％和８０．１±６．９％．在化学去交感神经的膜制备中，α－ＭｅＨＡ仅轻度降低组胺与膜受体的特异性结合量．结论：化学法切除交感神经后，丢失了一部分与［￣３Ｈ］组胺结合的受体蛋白，这部分定位于交感神经末梢的组胺受体，可以被α－ＭｅＨＡ竞争性结合，属于组胺Ｈ－３受体．
objective :To observe the specific
binding of[￣H]histamine on the
membrane fraction of guinea pig myocardium following chemical
sympathectomy by
6-hydroxydopamine. Metkod :The isolated guinea pig heart was
homogenized and membrane
fraction of myocardium was incubated with[￣3H]histamine for 30 minat
37℃. Then incubated
fluid received rapid filtration over glass fiber filter and after
dried ,filter membrane was placed
in a scintillator so as to measure radioactivity by liquid
scintillation spectrometry. Results :
Selec-tive H-3-receptor agonist α-methylhistamine (α-MeHA, 1 μmol
/L)could competitively
inhibit the specific binding of[￣3H]histamine on the membrane
fraction of myocardium ,
which decreased the specific binding quantity to 64.1±11.1%and
60. 5±4.1%of control
values respectively when the concentrations of[￣3H]his-tamine were
0.5 and 1 nmol/L.
Chemical sympathectomy by 6-OHDA could bring the specific binding
quanti-ty between
histamine and membrane receptors down to 77.3±6. 9 and 80.1±6.9%of
control values. While
α-MeHA caused only slight drop of the specific binding between
histamine and membrane
fractiion treated by sympathectomy. Conclusion :Chemical
sympathectomy can give riseto loss
of some presynaptic binding sites(or receptors) of[￣3H]Hihistamine
located on sympathetic
nerve terminals. α-MeHA can combine competitively with these
histamine receptors so they
might be H-3-subtype of histamine receptors.
出处
《第四军医大学学报》
1995年第5期324-327,共4页
Journal of the Fourth Military Medical University
关键词
心肌
交感神经末梢
突触
组胺H3受体
受体
histamine
H-3-receptors
(R)-α
-methylhistamine
[￣3H]histamine
myocardium
