摘要
给雄性ＢＡＬＢ／ｃ小鼠腹腔注射人重组白细胞介素－８（ｈｒＩＬ－８），能剂量依赖性地诱导中性粒细胞（Ｎｅｕ）向腹腔的游走。预先给小鼠皮下注射甾体类抗炎药地塞米松０．５～１２．０ｍｇ／ｋｇ，能显著抑制ｈｒＩＬ－８诱导的这一趋化过程，而预先皮下注射非甾体类抗炎药吲哚美辛２．５～４０ｍｇ／ｋｇ则无显著影响。具有钙拮抗作用的抗炎药粉防己碱６．２５～２５．０ｍｇ／ｋｇ和钙拮抗剂维拉帕米１．２５～５．０ｍｇ／ｋｇ，预先皮下注射也能剂量依赖性地抑制ｈｒＩＬ８诱导的Ｎｅｕ游走，提示ｈｒＩＬ－８在体内对Ｎｅｕ的趋化是一个钙离子依赖的过程。预先皮下注射亚胺环己酮１．５～１３．５ｍｇ／ｋｇ并不影响ｈｒＩＬ－８诱导的中性粒细胞游走，提示ＩＬ－８的趋化作用并不需要新合成蛋白质所介导。
A dosage-dependent neutrophil migration was observed after an injection of human recombinant interleukin-8(hrIL-8)into the peritoneal cavity of BALB/c mice,Pretreatment of the mice with subcutaneous injection of 0.5￣2.0mg/kg of dexamethasone significantly inhibited the neutrophily migration induced by hrIL-8 while pretreatment with indomethacin 2.5￣40.0mg/kg subcutaneously showed no effect.Pretreatment with tetrandine,(6.25￣25.0mg/kg),an anti-inlfammatory agent with antagonistic effect against calcium and verapamil(1.25￣5.0mg/kg),a calcium antagonist,were also effective to inhibit the neutrophil chemotaxis induced by hrIL-8 in a dosage-dependent manner.Pretreatment with cycloheximide(1.5￣13.5mg/kg subcutaneously),a protein synthesis inhibitor,resulted in no inhibition on neutrophil migration induced by hrIL-8.These facts indicate that IL-8 induces neutrophil migrationin vivo through a calcium-dependent mechanism and requires no new protein synthesis.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1995年第6期485-488,共4页
Journal of Third Military Medical University
关键词
白细胞介素
中性粒细胞
地塞米松
吲哚美辛
interleukin-8
neutrophil
dexamethasone
indomethacin
tetrandine
cycloheximide
