摘要
应用神经药理和电生理技术探讨了河豚毒素（Ｔｅｔｒｏｄｏｔｏｘｉｎ，ＴＴＸ）单克隆抗体８Ａ＿５对河豚毒素阻滞钠通道作用的影响。结果表明，ＴＴＸ单抗８Ａ＿５能有效地拮抗ＴＴＸ对直接电刺激诱发膈肌收缩和大鼠坐骨神经动作电位的抑制作用。全细胞式钳位记录观察到，当ＴＴＸ使成神经细胞瘤ＮＧ１０８细胞Ｎａ＋电流完全消失后，加入８Ａ＿５１ｍｉｎ，Ｎａ＋电流峰值恢复到正常的７９．４４％±４．２０％，５ｍｉｎ时，为７３．８９％±６．７４％；将８Ａ＿５和ＴＴＸ各１μｍｏｌ／Ｌ事先混合１ｈ后加入，１ｍｉｎ时Ｎａ＋电流峰值维持在给药前的８９．２６％±７．４１％。表明ＴＴＸ单抗８Ａ＿５无论以何种方式给药都能有效地拮抗ＴＴＸ对ＮＧ１０８细胞Ｎａ＋电流的抑制作用。
The effects of tetrodotoxin(TTX) monoclonal antibody(McAb)8A_5 on TTX blockade of sodium channel were studied with the use of neuropharmacological and electrophysiological techniques. It was found that McAb 8A_5 which was mixed with TTX for 1h before applying could effectively antagonize the inhibitory action of TTX on the contraction of the rat diaphragm and the action potentials of the sciatic nerve evoked by directly stimulating. In the experiment of the whole cell current recording, McAb 8A_5 itself could not affect the sodium current peak of the NG108 cell. After the sodium currunt peak of cells was completely abolished by TTX,treating the cell with McAb 8A_5 for 1 min and 5 min,it was restored to 79.44%±4.20% and 73.89±6.74%(n=5) of control values,respectively.When the cells were treated for 1 min with the mixture of McAb 8A_5 and TTX which were mixed for 1h before added,the sodium current peak was maintained at 89.26%±7.41%(n =4) of the control.These results indicate that TTX induced blockade of the sodium current peak could be powerfully antagonized by McAb 8A_5 no matter how 8A_5 is added.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1995年第6期531-534,共4页
Academic Journal of Second Military Medical University
关键词
河豚毒素
抗体
离子通道
tetrodotoxin
antibodies
monoclonal
ion channel,sodium
whole cell recording
