摘要
采用离体孵育脑薄片和放射免疫测定其分泌精氨酸升压素（ＡＶＰ）的方法，观察几种神经递质及其受体阻断剂对大鼠下丘脑薄片分泌ＡＶＰ的影响。结果：（１）大鼠下丘脑薄片对ＡＶＰ的基础分泌量为９．７５±２．５４ｐｇ／ｍｉｎ；（２）乙酰胆碱（１０－５ｍｏ１／Ｌ）刺激ＡＶＰ的分泌，使其分泌量增加到１５．７３±２．６８ｐｇ／ｍｉｎ，六烃季胺而非阿托品可阻断其作用；（３）肾上腺素（１０－４ｍｏｌ／Ｌ）强烈地刺激ＡＶＰ的分泌，使其分泌量增加到２０．１８±５．３９ｐｇ／ｍｉｎ，酚妥拉明而非普萘洛尔（心得安）可阻断其作用；（４）γ－氨基丁酸（ＧＡＢＡ，１０－５ｍｏｌ／Ｌ）抑制ＡＶＰ的分泌，使其分泌量减少到４．６４±２．３７ｐｇ／ｍｉｎ，荷包牡丹碱可阻断这种抑制作用。提示胆碱能、肾上腺素能和ＧＡＢＡ能神经可能参与对ＡＶＰ分泌的调节，相应地在ＡＶＰ神经内分泌细胞上可能至少含有ｎ－型胆碱能、α－型肾上腺素能和ＧＡＢＡＡ受体。
The arginine vasopressin(AVP) secreted from the hypothalamic slices of SD rats sectioned with vibratome and incubated in the static microchambers was measured by radioimmunoassay.The effects of neurotransmitters on AVP secretion and the influences of some of their antagonists were investigated. The results are as follows:(1)AVP was steadily secreted from the slices at a basal rate of 9.75±2.54 pg/min;(2) Acetylcholine(10-5mol/L) stimulated the secretion of AVP which was up to 15.73±2.68 pg/min.This stimulatory effect was blocked by hexamethonium but not atropine;(3) Epinephrine(10-4 mol/L) could significantly increase the secretion of AVP to 20.18±5.39 pg/min.Its effect was blocked by phentolamine but not propranolol;(4)γ-aminobutyric acid(GABA,10-5mol/L) decreased AVP secretion to 4. 64±2.37 pg/min,which was blocked by bicuculline.These results suggest that cholinergic ,adrenergic and GABAergic neurons might be involved in the regulation of AVP secretion, and on the AVP neurons there might be at least receptors for acetylcholine,epinephrine and γ-aminobutyric acid.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1995年第5期420-422,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
关键词
下丘脑
精氨酸
升压素
神经递质
hypothalamic slice
arginine vasopressin
acetylcholine
epinephrine
γ-aminobutyric acid
rats
