摘要
用多例提纯的ＢＪ－λ混合免疫ＢＡＬＢ／Ｃ小鼠的脾细胞与骨髓瘤细胞Ｓｐ２／０融合，获得３株稳定分泌抗人屹游离λ链ＭｃＡｂ的杂交瘤细胞株（ＡＨλＤ８、ＡＨλ１Ｃ１和ＡＨλ２Ｃ１）．它们分泌的ＭｃＡｂ均能与我室所有１７例ＢＪ－λ起强反应，但不与结合的λ型轻链及Ｋ型轻链反应，表明这３株ＭｃＡｂ可能是抗人Ｉｇ游离λ链共同抗原决定簇的．３株ＭｃＡｂ均属小鼠ＩｇＧ１亚类。添加ＥＬＩＳＡ表明它们识别相同的表位。用于检测正常人血清和尿中的游离λ链及多发性骨髓瘤患者的ＢＪ－λ均具有很好的特异性和敏感性．本文还讨论了有关的免疫问题．
Three stable hybridoma cell lines(AHros,AHAICI and AHnCI)secreting monoclonalanhbodies against human free lambda chains were obtained by fusion of SpZ/0 cells with splenocytesof BALB/c miCe bomunized with mixed pure lambda Bence--Jones proteins.All these McAbsshowed strong reachon with all 17 lambda Benocjones proteins isolated by us from different multiplemyeloma PatientS, but no reaction with combined lambda chains a7.i kappa chains.These results suggested that the 3 McAbs seem to be against common antigenic determinant of human free lambdachains. They all belonged to IgGI subclass and reacted with the same epitope. The preliminary testSshowed that these McAbs were good in both specificity and sensitivity for detection of free lambdachains in serum and urine of normal human and multiple myeloma Patients.Some problems ofinununization were discussed.
基金
福建省自然基金
关键词
免疫球蛋白
单克隆抗体
抗原决定簇
制备
immunoglobulin
lambda chain
antibodies
monoclonal
antigenic determinant
immunization
