摘要
本文应用ＨＰＶ－ＰＣＲ技术和ＤＮＡ杂交技术对７６例宫颈鳞癌标本进行了ＨＰＶ－ＤＮＡ的研究，发现ＨＰＶ－ＤＮＡ存在率为９０．７９％（６９／７６），主要类型为ＨＰＶ１６和１８。多酶切ＳｏｕｔｈｅｒｎＢｌｏｔ杂交证实在宫颈癌中９２．５６％的ＨＰＶ－ＤＮＡ发生变异，说明ＨＰＶ－ＤＮＡ的基因变异与其致癌作用密切相关。ＰＣＲ结果证实ＨＰＶ－Ｅ６片段在宫颈癌组织中常可出现，推测可能是致癌的关键片段之一。因此，检测宫颈癌组织中ＨＰＶ－ＤＮＡ的完整性、关键基因片段的存在与否。
In this paper,
HPV-PCR and DNA hybridization technique were used to study 76cases of cervical squamous
cancer. HPV-DNA was found in 69 cases(90.79%). The main typieswere HPV16 and l8. The
commonest type was HPV16. By Southern Blot,HPV-DNA was identifiedin 54 cases (92.56%)of
cervical cancer.Ihe results suggested that HPV was closely related to onco-genicity and that
HP-E6 fragment was one of key fragments to induce cervical cancer. So the examina-tion of
intactness and key fragment of HPV-DNA was significant to predict the prognosis of
HPVinfection.
出处
《白求恩医科大学学报》
CSCD
1995年第5期511-513,共3页
Journal of Norman Bethune University of Medical Science
