摘要
本文报告检测精子顶体酶活性的方法,是以Na一苯甲酰一DL一精氨酸一p一硝酰基苯胺(BNPNA)为底物,检测新鲜去精浆精子顶体酶活性。文中分析了实验的温度、时间、反应体系中精子浓度等因素对实验结果的影响,提出了最佳实验条件及注意事项。
Acrosin activity of
human spermatozoa without seminal plasma was measured with Na一benzoyl一DL一arginine一p
一nitroanilide(BAPNA)as substrate. We analyzed the effect of the ex-perimental temperature, time,
and sperm concentration in reaction system, and put forward the opti-mum experimental
conditions and the points for attention in this paper.
出处
《白求恩医科大学学报》
CSCD
1995年第5期465-468,共4页
Journal of Norman Bethune University of Medical Science
基金
国家自然科学基金
关键词
精子
顶体酶
检测
spermatozoa
acrosin
assay