摘要
啤酒废酵母（１０８－１０９细胞／ｍｌ）通气发酵后，经乙醇－氯仿沉淀，加热，调ＰＨ，（ＮＨ４）２ＳＯ４沉淀等除去杂蛋白质。最后经ＳｅｐｈａｄｅｘＧ－７５层析得到纯化的ＣｕＺｎ－ＳＯＤ，酶比活在１５００单位／ｍｇ蛋白以上，酶的分子量为１４８０００，由四个亚基组成，紫外最大吸收峰位于２５６ｕｍ处。Ａ２５６．４／２８０＝１．２２．
After fermentation by air of waste saccharomyces cerevisiae, predpitation of contaminat proteins with chloroform/ethanol, heat denataration, (NH4)2 SO4 salting out and control of PH value, the predpitated proteins were separated and the SOD contuining solution was purified by gel filtration (sephadex G-75). Said process to give a field of SOD with an activity of 1500 nits/mg, the molecular weight of Cu. Zn--SOD from yeast was 148000 consisted of four Subunits, the enzyme exhibited one absorption maximuxn in the Ultraviolet at 256. 4um, A 256. 4/A 280=1. 22.
出处
《安徽大学学报（自然科学版）》
CAS
1995年第2期88-92,共5页
Journal of Anhui University(Natural Science Edition)
