摘要
进行了由富马酸转氨生成Ｌ－天冬氨酸的酶反应动力学的初步研究，考察了酶活性的稳定性、底物浓度和酶浓度对酶反应速率的影响．针对高浓度底物对酶反应的抑制作用，提出了一个动力学模型方程：ｒ＝２１．４４ｅ０／（１＋０．０１０４４／Ｓ＋Ｓ／２．６３）－８９．７（Ｓ—０．０６４）２该模型方程在ｓ＝０．０２～０．１２ｋｇ／Ｌ范围内能较好地拟合实验数据。
The kinetics of the enzyme reaction, which converts fumaric acid into L--aspartic acid, has been studied. For the inhibitioh of the substrate in high concentration to the reaction, a model was derived from the reaction mechanism, which can be expressed as:Tg =21.44e0/(1+0.01044/S + S/2.63)-89.7(S-0.064)2The results calculated by this model were in good agreement with experimental values at S=0.02-0.12g/mL
出处
《南京化工大学学报》
1995年第A01期135-139,共5页
Journal of Nanjing University of Chemical Technology(Natural Science Edition)
