摘要
利用ＰＣＲ技术对４２对肝癌及癌旁正常肝组织、４个肝癌细胞株的Ｒｂ基因的两个数量可变的重复序列（ＶＮＴＲ）区进行分析，发现８例肝癌组织（１９．０２％）和２株肝癌细胞存在Ｒｂ基因的杂合性丧失（ＬＯＨ），表明Ｒｂ基因的缺失在肝癌的发生发展中起着一定作用。对ＰＣＲ－ＶＮＴＲ技术在快速检测Ｒｂ基因缺失上的价值进行了讨论。
The two variable number of tandem repeats(VNTR) regions of retinoblastoma gene(Rb gene) of 42 cases of hepatocellular carcinoma(HCC) tissues and 4 lines of HCC cells were analyzed by PCR technique.LOH of Rb gene were found in 8 of 42 HCC tissues and 2 of 4 HCC cell lines.This result suggested that the deletion of Rb gene should play a role in the carcinogenesis and development of hepatocellular carcinoma.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1995年第1期19-22,T004,共5页
Chinese Journal of Medical Genetics
关键词
视网膜母细胞瘤
易感基因
癌
肝肿瘤
Rb gene
Hepatocellular carcinoma
Variable number of tandem repeats
