摘要
本实验应用肌基膜管结合神经生长因子（ＮＧＦ）移植修复脊髓缺损。３０只大白鼠，于下胸段切断脊髓组织，造成５ｍｍ的脊髓缺损，分别进行：（１）１２只自体肌基膜管移植修复脊髓缺损；（２）１２只自体肌基膜管浸泡ＮＧＦ液移植；（３）６只脊髓切除后不作处理为对照组。术后１０～１２周分别取脊髓标本进行组织学评价，结果表明：肌基膜管结合ＮＧＦ移植组明显地好于单纯肌基膜管移植组。前者移植物与脊髓连接紧密，移植物中有较多的、排列整齐的神经纤维通过，腰段神经节内神经细胞很少发生变性坏死。后者移植物与脊髓断端存在空隙，移植物中有少量的、排列紊乱的神经纤维生长。经图像分析、轴突计数显示两级在统计学上有着显著差异。免疫组织化学方法与镀银染色进行比较，发现两种方法呈明显的相关性。本实验证明：肌基膜管结合ＮＧＦ移植用于脊髓横断的修复是可行的，肌基膜管作为桥梁为脊髓轴突再生提供了适宜的环境。ＮＧＦ具有刺激背根神经节神经元和脊髓轴突再生的能力和保护中枢神经元的作用。
AbstractLaminectomy was done in 30 wistar rats overT_9 and T_(10).Myelectomy of 5mm was done longitu-dinally on the thoracic spinal cord. The animalswere divided into 3 groups. (1)Autologous mus- cle basal lamina(MBL)grafts made withparaspinal muscle fibers,were implanted to thespinal cord gaps in 12 rats;(2)autologous MBLand nerve growth factor(NGF)graft were im-planted to the spinal cord gaps in 12 rats;(3)6rats served as controls,without implantation tothe spinal cord gaps. Neurological function wastested weekly for 12 weeks by the inclined planetechnique and the animals were sacrificed and thegrafted materials were procured for histological ex- amination. Tke results showed significantly bettermieroscopic appearance in the second group withimplantation of both MBL and NGF. Nerve fiberswere identified in the graft by immuno-histocyto-cliemical staining and silver staining. The distaldorsal root ganglion(DRG)cells were normal inthe second group and necrotie in the first and con- trol groups. There data,for the first time,showedthat MBL can provide a suitable environment foraxonal regeneration and NGF is able to enhancethe regeneration capacity of central nervous sys-tem; the use Of MBL and NGF graft for spinalcord repair is feasible.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1995年第1期32-35,T003,共5页
Chinese Journal of Orthopaedics
