摘要
用包裹DNA的金微弹轰击耳朵,将表达乙型肝炎病毒表面抗原(HBsAg)基因的重组质粒转入小鼠皮肤细胞,在小鼠血清中检测HBsAg及抗-HBs抗体。实验组8只小鼠中,有3只在基因转移3天后检测到HBsAg。14天再轰击1次。16天后2只鼠中检测到抗-HBs抗体。28天后4只鼠中检测出抗HBs抗体。对照组4只小鼠中HBsAg及抗-HBs抗体均未检测出。本实验为今后进一步分析乙肝病毒的遗传免疫奠定了实验基础,提供了实验依据。
A plasmid construct expressing hepatitis B virus surface antigen (HBsAg) was introduced into the skin cells of living mice by bombarding the ears with DNA-coated gold particles. HBsAg and anti-HBs antibody were assayed in sera of the mice following gene transfer.Three days after introduction of the plasmid,HBsAg appeared in 3 of the 8 treated mice. On day 14,the mice were rebombarded again and anti-HBs antibody was detected in two of the 8 treated mice 2 days after the second bombardment. On day 28, anti-HBs antibody was found in 4 of the 8 immunized mice. Neither HBsAg nor anti-HBs antibody was observed in the 4 controls.
出处
《标记免疫分析与临床》
CAS
1995年第3期147-149,157,共4页
Labeled Immunoassays and Clinical Medicine
基金
国家自然科学基金