摘要
用限制性内切酶TaqI和C4基因5 ̄'末端探针pAT-A检测58份湖北地区健康献血员基因组DNA的C4基因限制性片段长度多态性(RFLP),观察到7.0kb、6.0kb和5.4kb三种C4基因片段,它们共组成三种常见的C4基因RFLP表型,即A:7.05-4kb(频率为24.14%));B:7.0-6.0kb(频率为20.69%)和C:7.0-6.0-5.4kb(频率为55.17%,在所检测的样本中未观察到6.4kb的C4基因片段。
genomic DNA samples of blood donor were typed
for the C4 RFLP using restriction en-zyme Taq Ⅰ and C4 gene probe pAT-A. The samples
showed 3 polymorphic fragments(7.0kb,6.0kb and 5.4kb),and 3 common C4 RFLP phenotypes
were observed in the population,ie.A:7.0-5.4kb,B:7.0-6.0kb,C:7.0-6.0-5.4kb.Their frequencies
were 24.14%,20.69% and 55.17%respectivly. № 6. 4kb fragment wasfound with the samples.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1995年第2期84-86,共3页
Immunological Journal
基金
国家自然科学基金
国家教委优秀年轻教师资助