摘要
１９９０～１９９３年于青岛采用研究鱼类人工诱导雌核发育相类似的方法 ──紫外线照射精子、受精、温度或细胞松胞素Ｂ处理卵子方法，对中国对虾进行 人工雌核发育的研究。实验结果，有三批材料获得较好的效果：二批培育到蚤状幼 体，另一批培育到３～３．５ｃｍ幼虾。经受精细胞学观察，被照射的精子经受精，其核 有的不能原核化，有的虽能原核化，且能向中央迁移，但都不能与卵原核结合。实验 表明，这三批育出的个体是雌核发育的个体。
This work was designed to develop new techniques for selective breeding in shrimp using all-female shrimp populations. Artificially acquired unfertilized eggs of Chinese shrimp Penaeus chinensis were fertilized with UV irradiated sperm (lamp: 30W, distance: 20cm, the of irradiation: 105) of the same species and then treated with cold-shock (4℃, 30min) or cytochalasin-B (0. 25 mg/L, 10min) induce gynogenesis. Unfertilized eggs were obtained from naturally spawned females with blocked seminal receptacles or by dissecting ovaries from mature females. The procedures of retention of ho polar body by cold or cytochalsain B shocks were the same sa those commonly used to induce triploidy. Several different methods of sperm irradiation were compared, the most efficient one being the direct irradiation of sperm from the seminal receptacles of the females; a typical Hertwig effect was observed identification of gynogenesis was done by cytological examination of sectioned specimens from fertilized eggs and by observation of egg development. Two experimental batches yielded 14. 0% and 37. 2% hatched diploid gynogenetic embtyos, while the control yielded 0. 5% and 0% hatching of haploid embryos. Third experiment: A few of young shrimps were cultured with the gynogenetic embryos. Cytological examination demonstrated that the irradiated male nucleus did not fuse with the female nucleus to fome a zygote.
出处
《海洋科学》
CAS
CSCD
北大核心
1995年第3期35-41,共7页
Marine Sciences
基金
"七五""八五"国家攻关课题
关键词
中国对虾
人工诱导
雌核发育
四步诱导法
Chinese shrimp(Penaeus chinensis), Artificial gynogenesis, UV irradialed sperm
