摘要
本研究采用Ｆｅｕｌｇｅｎ染色和ＡｇＮＯＲ银染色，检测ＤＮＡ及ＡｇＮＯＲ物质，发现：ＤＮＡ含量Ⅲ级为甲状腺癌组＞非典型结节组＞结甲肿组＞对照组，而Ⅰ级则反之；ＡｇＮＯＲ均数为甲状腺癌组（９．２７±２：７２）＞非典型结节组（４．７８±１．６６）＞结甲肿组（３．１６±１．３８）＞对照组（３．０８±０．４１）；甲状腺癌组与各组间差异有显著意义（Ｐ＜０．０１），非典型结节组与结甲肿组、对照组间差异也有显著意义（Ｐ＜０．０１），而结甲肿组与对照组间则无差异（Ｐ＞０．０５）。作者认为Ⅲ级ＤＡＮ含量的细胞出现频率越高或／和ＡｇＮＯＲ均数越高，恶性的可能性就越大或分化程度越低；本研究提示ＡｇＮＯＲ的均数与ＤＮＡ含量具有显著的相关性，并与细胞增生的程度呈正比，故对鉴别良、恶性肿瘤及交界性病变有肯定价值。
Feulgen's staining and AgNOR silver staining were employed to detect the DNA content and AgNOR count. Our findings are as follows: DNA content Grade Ⅲ in thyroid cancer group>that in atypical nodular group>that in noduler goiter group>that in contrast group, while Grade Ⅰ is the opposite; AgNOR average count of the thyroid cancer group (9. 27±2. 72) >of the atypical nodular group (4. 78±1. 66) >of the nodular goiter group (3. 16±1. 38) >contrast group (3. 08±0. 41) ; there's marked difference between the thyroid cancer group and the other groups (P<0.01) and there's markable difference among the atypical nodular group, the nodular goiter group and the contrast group (P<0. 01) and there's no difference between the nodular goiter group and the contrast group (P>0. 05). This study suggests the marked corelation of the average count of AgNOR and DNA content and a direct ratio with cellproliferation, therefore, it is of affirmative velve in differetiating benign, malignant carcinoma and junctional lesion.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1995年第4期205-206,213,共3页
Cancer Research on Prevention and Treatment
基金
自治区卫生厅"青科"资助
关键词
甲状腺肿瘤
鉴别诊断
DNA
AGNOR染色
Thyroid cancer
atypical proliferation
nodular goiter
DNA content
AgNOR count
