摘要
作者采用ＤＮＡ电泳及荧光染色流式细胞仪（ＦＡＣＳ）分析方法，检测了凝集素依赖的ＬＡＫ细胞介导的肿瘤细胞死亡机制。在体外以白血病和实体瘤细胞系为靶细胞，在１μ／ｍｌ的凝集素美洲商陆（ＰＷＭ）存在下，人ＬＡＫ细胞在４小时之内就可诱导这些肿瘤细胞（Ｕ９３７细胞、Ｒａｊｉ细胞，ＳＷ１１１６细胞和Ｈｅｐ２细胞），出现以ＤＮＡ断裂成不连续的片断为特征的细胞调亡（ａｐｏｐｔｏｓｉｓ）现象。用ＲＮＡ及蛋白合成抑制剂预处理靶细胞并不能阻断其ＤＮＡ断裂。ＦＡＣＳ分析显示在ＰＷＭ存在下ＬＡＫ细胞诱导靶细胞凋亡的程度高于ＬＡＫ细胞单独使用时。而且实验提示ＬＡＫ细胞在与靶细胞相互作用的同时，也会部分地发生细胞死亡。以上结果表明诱导靶细胞凋亡是ＬＡＫ细胞在凝集素存在下杀伤肿瘤细胞的有效手段之一，由此为提高ＬＡＫ细胞疗效提供了新的实验依据。
Abstract By using DNA electrophoresis and
propidium iodide(PI) staining flow cytometry(FACS)analy-sis , we
studied the mechanisms of lymphokine-activated killer(LAK )
cell-mediated cytotoxicity. Inthe presence of pokeweed mitogen(PWM) ,
human LAK cells induced DNA fragmentation of twoleukemic cell
lines(U937 cells and Raji cells)and two solid tumor cell lines(SW1116
cells and Hep-2cells), a hallmark of apoptosis. The reactions were
carried out at the effector/target ratio of 1 : 1 andin 4 hr
coculture. Pretreatment with RNA and protein synthesis
inhibitors(actinomycin D and cyclo-heximide)did not prevent the
target cells from apoptosis. As the TNF-resistant tumor cell lines
suchas SW1116 cells and Raji cells were also triggered to apoptosis,
other factors than TNF would play therole. DNA-PI staining FACS
analysis also suggested that a part of LAK cells underwent apoptosis
tosome extent during incubation with target cells, The results
provide a new way to investigate themechanisms of cytotoxicity of LAK
cells and to enhance the efficacy of adoptive tumor therapy withLAK
cells.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1995年第4期245-248,共4页
Chinese Journal of Oncology
基金
国家自然科学基金
