摘要
为了探讨含Ｔ、Ｂ淋巴细胞双重激活表位的重组复合症疾抗原的免疫原性和保护作用，作者设计合成了编码恶性疟原虫红内期３个保护性抗原位点和２个外源性Ｔ细胞激活位点的复合基因ＨＧＦＣ，并构建了多连体复合基因ＨＧＦＣＡＣ。两种复合基因分别与表达载体ｐＷＲ４５０－１重组，并在大肠杆菌中表达出含外源蛋白和β－半乳糖苷酶部分氨基酸的融合蛋白（分子量分别为６５０００和７７０００），表达量为３５％。表达产物可与小鼠及兔抗恶性疟原虫抗体发生特异性免疫反应。用纯化的融合蛋白免疫家兔制备的免疫血清可特异地识别恶性疟原虫抗原，并对疟原虫体外生长有显著抑制作用。抑制程度与免疫血清浓度及作用时间呈正相关，在２０％浓度下作用７２小时，抑制率可达８２％，并引起疟原虫发育不良和死亡。研究结果说明，制备的恶性疟原虫重组复合抗原具有免疫原性和一定的免疫保护作用，可作为恶性疟疫苗候选物。
hybrid gene named
HGFC coding three protec-tive antigenic epitopes of Plasmodium falciparum andtwo exogenous
T cell activating epitopes was designedand synthesized. A multicopy hybrid gene named
HGFCAC was also constructed. The two genes were clonedinto expression vector pWR450-1
and the hybrid fusionproteins containing forgine antigens and β- galactosidasewere expressed
in E. Coli. The molecular weights of thefusion proteins were 65KDa and 77KDa respectively.The
expression rate was about 35% of total bacterialproteins. The fusion protein could react
specifically withmouse and rabbit antibodies against antigens of Plas-medium falciparum. The
rabbit immune serum againstthe purified fusion protein could specifically recognizethe antigens
of Plasmodium falciparum and effectivelyinhibit the in vitro development of the parasites. The
in-hibitory capacity of the immune sera to parasite invasionwas enhanced as the amount of the
sera increased andthe incubation time of the sera with the parasites wasprolonged. After 72h
incubation at 20% concentrationwith the parasites, the serum suppressed the multiplica-tion of
parasite to a level of 82% and caused degenera-tion and death of theparasites. The results
indicatedthat the recombinant hybrid antigen of Plasmodium fal-ciparum has immunological
activity and protectivity. Itis probably a candidate malaria vaccine.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1995年第6期325-328,共4页
National Medical Journal of China
关键词
恶生疟原虫
疟原虫
重组蛋白类
疫苗
免疫活性
Plasmodium
falciparum Recombi-nant proteins Vaccine Genes lmmune activity
