摘要
为探讨细胞间粘附分子－１（ＩＣＡＭ－１）与肾小球炎症的关系，根据肾小球内细胞增生的程度将６４例不同类型肾小球疾病患考分为Ａ组（肾小球炎症不明显或很轻微）、Ｂ组（肾小球有肯定的细胞增生）、Ｃ组（以硬化为主的肾小球晚期病变），观察肾组织中ＩＣＡＭ－１的表达。结果（１）肾小球内ＩＣＡＭ－１表达水平日组显著高于Ａ组（Ｐ＜０．００１）；Ｃ组明显低于Ｂ组（Ｐ＜０．０５）。（２）Ｂ组患者肾小管表达ＩＣＡＭ－１的阳性率明显高于Ａ组（Ｐ＜０．０５）；Ｃ与Ｂ组间差异无显著意义（Ｐ＞０．０５）。（３）伴有ＩＣＡＭ－１阳性间质浸润细胞组肾小管表达ＩＣＡＭ－１的阳性率高于不伴有ＩＣＡＭ－１阳性间质浸润细胞组（Ｐ＜０．００５）；（４）原位杂交结果证实ＩＣＡＭ－１蛋白质表达部位与其ｍＲＮＡ表达部位一致。提示ＩＣＡＭ－１在人类肾小球疾病中具有重要作用。
e conducted immunocytochemical analysis with insitu hybridization
technique to investigate the presenceand role of intercellular adhesion molecule-1(ICAM-1)in
normal and diseased kidnevs. A total of 64 renalbiopy specimens were classified in three
groups accordingto the degree of cellular proliferation in glomerulus.Group A presented no or
mild proliferation (n=38 ).Group B showed definite proliferation (n=21).GroupC had glomerular
sclerosis (n=5). The ratio of casesexhibiting increased glomerular ICAM-1 expression inlupus
nephritis( 9/10) was significantly different fromthat in minimally changed disease (1/5)(P=0.
017).Glomerular ICAM-1 expression level correlated wellwith the cellular proliferation and
infihration inglomerulus (B&A: P<0. 001; C&B: P<0. 05). Thepercentage of cases with tubular
ICAM-1 positivity ingroup B (81. 0%) and group C (100%) was greaterthan that in group A (52.
6%), which was also corre-lated with that of interstitial cell infiltration. In situ hy-bridization of 9
renal biopsies with digoxigenin labelledoligo probe proved immunocytochemical findings.
Weconclude that glomerular ICAM-1 expression level iscorrelated with inflammatory degree of
glomerulus andthat tubular ICAM-1 expression is associated with in-terstitial cell infiltration.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1995年第4期201-203,共3页
National Medical Journal of China
基金
国家自然科学基金
