摘要
采用原位杂交方法检测了急性白血病骨髓活检标本及细胞株中ｂｃｌ－２基因表达水平。结果２２例白血病患者骨髓中２１例（包括髓系和淋巴系）存在ｂｃｌ－２ｍＲＮＡ高表达；６种白血病细胞株中５种ｂｃｌ－２ｍＲＮＡ阳性（Ｍｏｌｔ－４例外），它们分别为ＣＥＭ、Ｒａｊｉ、ＨＬ－６０、Ｕ９３７及Ｋ５６２。表明造血系统肿瘤中广泛存在ｂｃｌ－２基因转录水平激活，其基因产物可能通过抑制细胞凋亡过程而影响急性白血病细胞的生物学特性。
cl-2 mRNA was detected in bone marrow trephinebiopsies from leukemia patients and leukernic cell linesby in situ hybridization. In 21 of 22 biopsies, most ofthe leukemic cells (myelogenous or lymphocytic )ex-pressed high levels of bcl-2 mRNA. BclZ mRNA wasdetectable not only in lymphoid cell lines (Raji , CEM)but also in myeloid cell lines(K562 , HL-60 , U937)except for Molt-4 (T-cell leukemia). These resultssuggested that bcl-2 gene was broadly activated transcriptionally in various hematopoietic neoplasms , andbcl-2 transcripts might influence the biological hehay-ior of leukemic cells by inhibiting apoptosis.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1995年第12期631-633,共3页
Chinese Journal of Hematology
基金
国家自然科学基金
