摘要
ＲＡ５３８基因是用维甲酸在体外迫使食管癌细胞系发生终未分化，建立分化前后的ｃＤＮＡ文库，进行递减杂交后分离所得。转移到食管癌细胞后可抑制其增殖，引起细胞脱落和死亡。测定和分析ＲＡ５３８ｃＤＮＡ序列，靠近５＇端有一个可能的蛋白编码区。为研究ＲＡ５３８基因的功能，用多聚酶链反应技术扩增分离了这个可能编码区ＤＮＡ序列，借逆转录病毒载体转入白血病细胞，发现对ＨＬ－６０细胞的生长有明显的抑制作用，提示它在肿瘤基因治疗中有潜在应用价值。
A538 gene was isolated from the EC8712esophageal cancer cell line treated with retinoic acid . Itsuppresses the proliferation of esophageal cancer cell invitro. There is a potential protein ceding fragment nearits 5' end. We amplifed and isolated this fragment byPCR niethod . and cloned it into a retrovirus vector . Af-ter introducing the recombinant retrovirus vector intoHL-60 cell by lipofectin, we find it can suppress thegrowth of HL-60 cell effectively. The result suggeststhat the protein coding fragment of RA538 gene may beused for gene therapy of cancer.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1995年第5期229-231,共3页
Chinese Journal of Hematology
关键词
基因治疗
逆转录病毒
维甲酸
白血病
抑癌基因
Gene therapy RetrovirusRetinoic acid Leukemic cellTumor suppressing gene
