摘要
应用ＤＮＡ多聚酶链反应（ＰＣＲ）技术对急性淋巴细胞白血病（ＡＬＬ）进行了Ｔ细胞受体（ＴＣＲ）δ基因重排的研究，并获得了３个ＡＬＬ患者ＴＣＲδ基因的Ｖ－（Ｄ）－Ｄ结合部顺序（Ｎ顺序）。证明ＡＬＬ，特别是Ｂ细胞ＡＬＬ中，ＴＣＲδ基因不完全重排的发生率比较高，最常见的两种重排类型为Ｖδ＿２－（Ｄ）－Ｄδ＿３和Ｄδ＿（１／２）～Ｄδ＿３。δ基因的不完全重排是早期淋巴分化调控中一个重要的内容。δ基因Ｖ－（Ｄ）－Ｊ结合部的Ｎ顺序具有高度的特异性，可作为检测ＡＬＬ微小残留病变的特异性克隆标志。
he rearrangement of T cell receptor (TCR )δ genein a group of patients with acute lymphoblastic leukemia(ALL) was studied by polymerase chain reaction(PCR),and the three V-(D)-D junctional sequences ofTCRS gene were obtained. The results showed that inALL, especially in B lineage ALL,the incidence of in-complete rearrangement of TCRS gene was quite high.Among these incomplete rearrangement, Vδ2- (D)-Dδ3and Dδ1/2-Dδ3 are the two prevalent patterns. Incom-plete rearrangement of TCRδ gene is an important eventin the regulation of early lymphoid differentiation.Meanwhile, the N sequence of V-(D)-J junctional re-gion in TCRS gene is highly specific for each lymphoryteclone, and may be used as a clonality marker to detectminimal residual disease (MRD) in ALL.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1995年第1期9-10,共2页
Chinese Journal of Hematology
基金
国家自然科学基金
卫生部项目基金
卫生部青年基金
关键词
淋巴细胞
白血病
T细胞
受体
基因
基因重排
Leukemia, lymphoblastic, acute Tcell receptor (TCR)δ gene Incom-plete rearrangement
