摘要
采用原位PCR技术对22例石蜡包埋肝组织中HBV DNA进行检测,并与提取组织DNA PCR技术及原位杂交技术进行比较。结果表明,22例标本经原位PCR检测有9例为阳性;经提取组织DNA PCR检测有7例阳性,两者敏感性相近,符合率达80％;经原位杂交检测有4例阳性,其敏感性只及原位PCR的44％。提示原位PCR是项快速、敏感及特异性较高的检测技术。
Twenty two slides of liver tissues hepatitis B virus DNA embedded in paraffin were detected by in situ polymerase chain reaction (ISPCR). and compared with the method of PCR by extracted DNA and in situ hybridization (ISH). The results showed that there were nine and seven positive slides by ISPCR and PCR, respectively. They had similar sensitivity. Only four positive slides were seen by ISH. It suggests that ISPCR might be a rapid,sensitive and specific technique method.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1995年第2期140-142,共3页
Chinese Journal of Microbiology and Immunology
关键词
聚合酶链反应
ISPCR
原位杂交
肝细胞
In situ polymerase chain reaction
Extracted DNA polymerase chain reaction j In situ hybridization
