摘要
应用非竞争ＥＬＩＳＡ和ＥＬＩＳＡ相加试验对８株抗肾综合征出血热病毒（ＨＦＲＳＶ）大鼠单克隆抗体（ＭｃＡｂ）进行了抗体亲和力和抗原结合位点的测定。结果提示这些ＭｃＡｂ至少可识别ＨＦＲＳＶ核壳蛋白（ＮＰ）上６种不同抗原决定簇，并且表明ＮＰ上可能存在ＨＦＲＳＶ组、亚组、型或亚型抗原决定簇。ＭｃＡｂ亲和常数测定的高低排序结果为ＫＦ１２＞ＤＥ１１＞Ｘ１０＞ＣＨ１０＞ＩＧ４。
Relative affinity of the five rat monoclonal antibodies anti-HFRSV were determined by non-competive ELISA.The results showed that the affinity of the McAbs could be ranked as following KF12>DF11<X10>IG4. Combiniging these McAbs immunobiological activites and antigenic analysis, it indicated that McAb KF12, DF11 and X10 are more worthy for application in HFRS studies. Antigenic sites of eight rat McAbs against NP of HFRS virus were measured by ELISA test. It was found that eight McAbs recognized six different antigenic sites on NP. Combiniting antigenic analysis of these McAb, it suggested that the group, subgroup, type and subtype antigenic determinats to HFRS virus may be located on NP.
出处
《中华实验和临床病毒学杂志》
CSCD
1995年第2期119-121,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
出血热病毒
单克隆抗体
肾病综合症
抗原位点
Hemorrhagic fever with renal syndrome virus
Rat
Monoclonal antibody
Antigenic site
Affinity
