摘要
对肾综合征出血热病毒（ＨＦＲＳＶ）经呼吸道传播的有关机理进行了一些实验研究。结果表明：大白鼠吸入ＨＦＲＳＶ气溶胶后，９５％沉积于呼吸道，而进入消化道仅５％；吸入后５～１４ｄ感染鼠肺泡内单个核细胞增多占７５％～８０％，泡浆内可检测到ＨＦＲＳＶ颗粒，检测高峰在感染后７～１４ｄ、２１ｄ消失；肺脏的病理改变主要在ＨＦＲＳＶ气溶胶吸入感染后的第１４～２１ｄ，电镜下表现为血液循环障碍，血管内皮细胞及细胞内线粒体严重损伤，胶原纤维有淀粉样病变和肺泡Ⅱ型细胞的板层体严重损伤，２８ｄ后部分组织结构开始恢复；用聚合酶链反应技术从感染鼠的肺、心、脾、肝和肾脏检测到ＨＦＲＳＶ－ＲＮＡ。用模拟现场研究发现，在攻毒组实验动物检出ＨＦＲＳＶ抗原后的第１５ｄ，从实验组动物中检出ＨＦＲＳＶ，表明两者有一定的伴随关系，并从实验动物室内气溶胶中分离到２株ＨＦＲＳＶ。这些研究表明ＨＦＲＳＶ可以气溶胶形式经呼吸道感染。这对ＨＦＲＳＶ传播途径的研究提供了重要的理论依据。
This paper reports our experimental research on the mechanisms of transmission of Hantavirus by aerosol. The results showed that 95 percent of the Hanta virus was retained in the respinatory tract, only 5 percent in the digestive tract, when the rats inhaled HFRSVS. The mononuclear phagocyte of the infected rats in the alveoli inreased to 70-80 percent, from 5 to 14 days after inhalation. HFRSV particles could be detected in pulmonary. The highest detected peak was at 7-14 days after infection, which disappeared 21 days after infection. The pathologic changes of the lungs were mainly at 14-21 days after HFRSV was inhaled. The inpediment of blood circulation could be found through electron microscropic examination. The endothelial cells of blood vessel and mitochondria showed seriously damaged. The structures of the tissues began to recover after 28 days. And HFRSV-RNA could be detected in lung, heart, spleen, liver and kidney of the infected rats by PCR amplicatious. Through minic field research, we found that 15 days after infection the antigen of HFRSV was detected from the experimental animals and also from the control group, and two strains of HFRSV were isolated from alerosol in the rooms of experimental animals. This research showed that HFRSV could infect animal by alerosol.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1995年第2期122-125,共4页
Chinese Journal of Experimental and Clinical Virology
关键词
传播
出血热病毒
气溶胶
肾病综合征
Hemorrhagic fever with renal syndrome virus
Aerosol
Transmission
