摘要
目的：为探明锂抑制甲状腺细胞合成激素的机理，研究了锂对体外培养甲状腺细胞（ＦＲＴＬ－５细胞）摄碘功能的影响。方法：ＦＲＴＬ－５细胞经无促甲状腺激素（ＴＳＨ）培养７天后，加入锂（０．５～１０ｍｍｏｌ／Ｌ）和ＴＳＨ（５０，１００ｍＵ／Ｌ）培养２４小时，然后换用含３．７ｋＢｑＮａ１２５Ｉ和１０ｎｍｏｌ／ＬＮａＩ的ＨＢＳＳ液。终止反应后消化细胞并测定细胞内１２５Ｉ量。结果：ＴＳＨ刺激ＦＲＴＬ－５细胞后，细胞内１２５Ｉ迅速升高，３０分钟达最高峰，延长反应时间其摄取１２５Ｉ量无显著变化；过氯酸钠可迅速降低细胞内１２５Ｉ达９０％以上。锂明显抑制ＴＳＨ刺激的ＦＲＴＬ－５细胞摄取１２５Ｉ，对基础状态的ＦＲＴＬ－５细胞无影响。２－１０ｍｍｏｌ／Ｌ锂减少ＦＲＴＬ－５细胞摄取１２５Ｉ超过５０％。结论：锂抑制甲状腺细胞的摄碘功能。
PURPOSE In order to elucidate the mechanism of action of lithitm on the thyroid gland,we studied the effeCt of lithium on the 125I uptake in thyroid cells in vitro.METHODS After culturing FRTL-5 cells 7 days in a medium lacking TSH, the cells were incubated with various concentrations of lithium(0.5-10mmol/L)and TSH (50,100mU/L) for 24 h.Iodide uptake was initiated by adding 500μl buffered HBSS containing 0.1μCi carrier-free Na125I and 10nmol/L Nal.After incubation,the cells were dissolved and their radioactivities counted.RESULTS The 1251 uptake in FRTL-5 cells rose to peak level in 30 minutes.Prolonging the incubation period did not change the iodide uptake.During the peak phase odium perchloride suppressed the 125I uptake more than 90%.The iodide uptake induced by TSH was inhibited at 1mmol/L lithium (P<0.05).More than 50% inhibition was observed at 2-10mmol/L lithium.There was no effect of lithium on the iodide uptake in the hasal condition of PRTL-5 cells (lacking TSH).CONCLUSION Lithium inhibits iodide uptake in thyroid cells in the presence of TSH.
出处
《中华核医学杂志》
CAS
CSCD
北大核心
1995年第4期242-244,共3页
Chinese Journal of Nuclear Medicine
