摘要
目的:探讨儿童咽喉乳头状瘤(JOP)与HPV6、HPV11病毒感染及患儿免疫功能的关系.方法:用荧光定量聚合酶链反应(FQ-PCR)检测130例JOP组织病毒DNA,并对其中68例采用流式细胞仪进行细胞免疫功能指标CD3、CD4、CD8检测.结果:130例标本中HPV6和HPV11病毒的检出率为88.46%(115/130),平均拷贝值为5.68±2.65.68例患儿CD3、CD4、CD8百分平均值分别为62.73±8.63、30.54±7.05、26.08±6.93,与对照组比较,差异无统计学意义.结论:FQ-PCR方法简便、准确、特异性强、灵敏度高、定量范围宽,可测定病原体数目为101～1010的不同程度,对JOP病毒的临床诊断、治疗及疗效观察有重要指导作用.
Objective:To investigate the relationship between juvenile-onset respiratory papillomatosis(JOP) and human papilloma virus (HPV6,HPV11),and the immune function of patients.Method:Fluorescence quantitative PCR (FQ-PCR) which combines PCR and fluorescence probe hybridization was used to detect HPV6,HPV11 DNA in 130 cases. Of these , 68 cases were used Flow Cytometry to measure CD3,CD4,CD8.Result:One hundred and fifteen of 130 cases were HPV6,HPV11 DNA positive, the average copy was 5.68±2.65. For 68 cases,the average percent of CD3,CD4,CD8 were 62.73±8.63,30.54±7.05,26.08±6.93,respectively.To compare with control group, there was not statistical significance.Conclusion:FQ-PCR is a convenient, accurate and specific method which can quantify the amount of pathogenic germs from 10~1 to 10~10,and is also a reliable factor to predicate clinical diagnose and cure.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2005年第15期694-696,共3页
Journal of Clinical Otorhinolaryngology
关键词
喉肿瘤
乳头状瘤
乳头状瘤病毒
人
Laryngeal neoplasms
Papilloma
Papillomavirus,human
