摘要
目的:建立癌细胞系是非常困难的工作,通常需要经过多次失败才能成功,本研究旨在通过对传统建立癌细胞系方法的改进以探讨一种容易建立癌细胞系的方法。方法:将获取的癌瘤标本分成两部分,一部分按传统的方法直接培养,另一部分首先将肿瘤标本接种在裸小鼠皮下形成皮下移植瘤,当移植瘤直径达到1cm时,取出肿瘤再在裸小鼠体内连续传代,从第3代开始将移植瘤的一部分做细胞培养。结果:按传统方法培养没有获得所需要的癌细胞,经过裸小鼠传代后的肿瘤组织再做培养很容易获得第1代肿瘤细胞,并且培养周期较短。利用这种方法我们成功地建立了一株舌癌细胞系TSCCa。结论:经过裸小鼠体内连续传代后的肿瘤组织较其原肿瘤组织容易建立癌细胞系。
Objective: To improve the traditional method of establishing cancer cell line in order to search out an easy method of establishing cancer cell line. Methods: The tumor samples were divided into two parts: one part were used directly for tissue culture by traditional method, the other part were implanted firstly to the subcutaneous of nude mice and formed subcutaneous tumor. When the diameter of the subcutaneous tumor reached 1 cm, the tumor was subcultured continuously in vivo in nude mice. Finally, the tissues from the third generation of subcutaneous tumor were extracted for culture in vitro. Results: The cancer cells by traditional method could not be acquired, while it was much easier to gain the first generation cancer cells, and the culture period of the first generation was shortened by the modified method. By this method, one cell line of human tongue squamous cell carcinoma was established successfully. Conclusion: It is easier to establish cancer cell line from the tumor tissue subcultured continuously in nude mice than from the primary tumor.
出处
《武汉大学学报（医学版）》
CAS
2005年第4期494-496,共3页
Medical Journal of Wuhan University
关键词
口腔癌
细胞系建立
改良方法
Oral Cancer
Establishment of Cell Line
Modified Method
