摘要
目的观察大黄素对骨髓基质细胞向脂肪细胞方向分化的影响,以探讨大黄素对骨质疏松发生时伴随的脂肪增多现象中所起的作用。方法16只3mon龄SD大鼠随机分成4组:对照组、激素组、复方碳酸钙治疗组、大黄素治疗组。给药90d后收集骨髓基质细胞。应用油红O染色法及RTPCR的方法判断是否成功诱导骨髓基质细胞向脂肪细胞方向分化,同时观察大黄素对骨髓基质细胞成脂分化后脂肪细胞的数目和脂蛋白脂酶mRNA表达的影响。结果体内灌胃给予泼尼松的激素组大鼠的骨髓基质细胞体外培养时分化为脂肪细胞的能力明显增强。复方碳酸钙组和大黄素组大鼠骨髓基质细胞分化为脂肪细胞的能力较激素组明显下降,其中大黄素下调脂蛋白脂酶mRNA表达的作用强于复方碳酸钙。结论体内给予糖皮质激素后,骨髓基质细胞体外培养时自发向脂肪细胞方向分化的能力和经体外诱导后向脂肪细胞方向分化的能力都明显增强,大黄素可能有对抗糖皮质激素成脂诱导的作用。
Aim To investigate the effects of emodin on the differentiation of MSCs into adipocytes in vitro. Methods We used the MSCs isolated from the intact rats and rats treated with either prednisone or other drugs. Three-month old SD male rats received 3 mg·kg -1·d -1 of prednisone oral gavage, or prednisone plus emodin at dose of 270 mg·kg -1, or prednisone plus compound recipe calcium carbonate. At the endpoint the MSCs were isolated by density-gradient centrifugation and cultured. The adipocytes were stained with Oil-red-O stain and counted, and mRNA expressions of Lipoprotein Lipase(LPL) were examined by RT-PCR. Results Through density-gradient centrifugation isolation and subculture attachment selection, the cultured MSCs became more purified. Emodin prevented adipogenic differentiation of MSCs in prednisone-treated rats by decreasing adipocyte number and down-regulating LPL mRNA expression. Emodin prevented adipogenesis of MSCs more potently than compound recipe calcium carbonate. Conclusion Emodin provided more predominant opportunities with which to prevent adipogenic differentiation of MSCs isolated from the rats treated with prednisone compared with compound recipe calcium carbonate.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第7期842-847,共6页
Chinese Pharmacological Bulletin
基金
广东医学院青年基金资助项目(NoXQ0307)
关键词
大黄素
骨髓基质细胞
脂肪细胞
分化
emodin
bone marrow stromal cells
adipocyte
differentiation
