摘要
目的了解上海地区2001～2003年淋病奈瑟菌(NG)对抗生素的敏感性情况及变化趋势。方法采用琼脂稀释法测定青霉素、头孢曲松、大观霉素、环丙沙星、四环素对742株NG的最小抑菌浓度(MIC)。用纸片酸度法检测β内酰胺酶菌株。用碱裂解方法抽提质粒。结果742株NG检出产青霉素酶菌(PPNG)342株(46.09%);质粒介导高度耐四环素(TRNG)166株(22.37%);耐环丙沙星718株(96.77%);耐大观霉素1株;未发现对头孢曲松耐药菌株;超过20%的菌株同时对青霉素、四环素和环丙沙星耐药;对35株菌株作了质粒分型,含耐药质粒检出率97%,分别带有5.4和7.4kb耐药性质粒。结论淋球菌对青霉素、四环素和环丙沙星3种药物分别有较高比例的耐药率。上海地区治疗淋病应首选大观霉素和头孢曲松。
Objective To survey the susceptibility and trend of Neisseria gonorrhoeae (N.gonorrhoeae) strains to antibiotic agents in Shanghai from 2001 to 2003. Methods 742 N.gonorrhoeae strains from clinical patients were randomly collected and observed for its susceptibility to five antibiotics.Agar dilution method was used to detect minimal inhibitory concentration (MIC) of anti-microbial agents:penicillin,tetracycline,spectinomycin,ciprofloxacin and ceftriaxone.β-lactamase testing was perforomed by acidometric method.The plasmid DNA was extracted by alkaline cleavage method. Results 342(46.09%) strains were penicillinase-producing N. gonorrhoeae (PPNG);166(22.37%) strains were high-level tetracycline resistant N.gonorrhoeae (TRNG);718(96.77%) strains were resistant to ciprofloxacin;1 strain was resistant to spectinomycin;No resistant strain to ceftriaxone was found.Multi-resistant (>20%) strains to penicillin,tetracycline and ciprofloxacin simultaneously were detected.35 strains were detected for plasmid map,34 trains showed 5.4 and 7.4 kb plasmid respectively. Conclusions The results indicate that at present,spectionmycin and ceftriaxone are the good choice for the treatment of gonorrhea.
出处
《检验医学》
CAS
北大核心
2005年第4期293-296,共4页
Laboratory Medicine
基金
上海市卫生局科研基金资助项目(1424)
关键词
淋病奈瑟菌
最小抑菌浓度
敏感性
质粒
Neisseria gonorrhoeae
Minimal inhibitory concentration
Susceptibility
Plasmid
