摘要
本研究观察了大肠杆菌内毒素对大鼠离体灌流肺的氧化性损伤作用，并探讨了中药７６４－３对该损伤的保护作用。结果发现单纯离体灌流肺给予内毒素刺激未能引起肺动脉压升高，这与在体情况下的反应不同。内毒素组的肺泡灌洗液中蛋白质含量和肺组织湿干重比值分别比其它组为高（Ｐ＜０．０５），该组肺组织匀浆和肺泡灌洗液中丙二醛（ＭＤＡ）含量也显著高于其它组（Ｐ＜０．０１）。中药７６４—３能够显著地减轻肺水肿（Ｐ＜０．０５），降低灌洗液和肺组织ＭＤＡ水平（Ｐ＜０．０１）。结果提示大肠杆菌内毒素对肺组织的损伤作用部分是通过刺激产生氧自由基的途径实现的。中药７６４－３具有较强的抗氧化能力，对肺组织具有较好的保护作用。
The role of oxygen free radicals in injury of
isolated perfused rat lungs induced by E.coliendotoxin and the
protective effect of 764-3 on such injury were investigated.764-3 is
a Chineseherb medicine extracted from Radix Salviae Miltiorrhizae.
There were 4 groups in the experiment:A, control; B.
endotoxin(ET,5mg/L);C. ET+764-3(0. 1g/L);D。 ET+DMSO(150mmol/L).The
perfusion lasted 90min. The results showed that the pulmonary
arterial pressure did not in-crease after stimulation with ET, being
different from research in vivo.In group B,wet/dryweight ratio of
lung tissue and the protein content in bronchio-alveolar lavage fluid
(BALF) werehigher than those in other
groups(P<0.05).Malondialdehyde(MDA) contents in BALF andlung tissue
in group B were significantly higher than that in group A,C and D
respectively(P<0.01).764-3 prevented lung edema formation(P<0.05)and
MDA increase in BALF andlung tissue(P< 0.01) induced by endotoxin.
These results suggest that oxygen free radicals maybe one of the
causes of lung injury induced by endotoxin and 764-3 may be a
potent antioxidant and can protect tissues from oxygen free radical
injury.
出处
《中国应用生理学杂志》
CAS
CSCD
1995年第3期225-228,共4页
Chinese Journal of Applied Physiology
基金
国家自然科学基金资助课题
关键词
中药764-3
离体灌流肺
肺损伤
内毒素
Chinese herb medicine 764-3
isolated perfused rat
lung
acute lung injury
E.coliendotoxin
Radix Salviae miltiorrhizae
