摘要
目的探讨大鼠肝缺血再灌注损伤早期肝内载脂蛋白MmRNA(apoMmRNA)及血浆apoM的表达。方法建立大鼠肝脏缺血再灌注损伤模型。健康雄性SD大鼠40只随机分成5组,每组8只:假手术组(对照组);IR1组(灌注0.5h);IR2组(灌注1.0h);IR3组(灌注2.0h);IR4组(灌注3.0h)。缺血再灌注组的缺血时间统一为1.0h。检测血浆谷丙转氨酶水平(ALT)、肝组织病理变化、血浆apoM蛋白及肝组织apoMmRNA。结果血浆ALT的水平随着灌注时间的延长而升高,肝组织损伤随着灌注时间的延长而逐渐加重。肝组织apoMmRNA的表达则先有一过性下降(灌注0.5h组),此后随着灌注时间的延长其表达明显增强。血浆apoM蛋白有相同的变化趋势,但其表达在灌注2.0h才有上升。结论在肝缺血再灌注损伤过程中,肝脏apoMmRNA的表达和血浆蛋白水平有迅速、明显的变化,提示apoM可能具有急性时相反应蛋白的特性。
ObjectiveTo investigate the expression of hepatic apolipoprotein M (apoM) mRNA and serum apoM during early stage of hepatic ischemia reperfusion injury in rats. MethodsRat models of hepatic (ischemia)-reperfusion injury (IRI) were established. Forty healthy male SD rats were randomly divived into five groups(each group containing eight rats):Sham-operation group(control); IRI groups, animals (experienced) 1 hr ischemia and then followed by various reperfusion intervals(0.5,1.0,2.0 and 3.0 hrs, respectively). ResultsSerum alanine-aminotransferase (ALT) levels and the liver injury degree (histologically) were increased with the prolongation of hepatic reperfusion time. The apoMmRNA level in liver was markedly decreased in the group that had reperfusion for 0.5hr compared to the sham group. However, it increased gradually in groups that had reperfusion for 1hr to 3hrs. Serum apoM protein level showed a (similar) tendency with apoMnRNA, but it increased after reperfusion for 2 hrs. ConclusionsIt is concluded that apoM expression pattern shows rapid and significant changes during hepatic ischemia-reperfusion injury, which suggests that apoM might have the characteristic of acute phase reactive protein.
出处
《中国普通外科杂志》
CAS
CSCD
2005年第7期501-504,共4页
China Journal of General Surgery
关键词
肝疾病
再灌注损伤
疾病模型
动物
载脂蛋白M
<Keyword>Liver Diseases
Reperfusion Injury
Disease Models,Animal
Apoliprotein M
