摘要
【目的】探讨辛伐他汀(Simvastatin,Sim)对新生SD大鼠心脏成纤维细胞(CFs)DNA合成的影响,为防治高血压心肌纤维化提供理论依据。【方法】采用胰酶消化、差速贴壁法培养新生SD大鼠CFs,以3HTdR掺入法测定DNA合成,观察不同浓度Sim分别作用不同时间对CFsDNA合成作用的影响。【结果】①CFs的3HTdR掺入率随着Sim干预浓度的增加而降低,其中10-6mol/LSim和10-5mol/LSim组的3HTdR掺入率分别为(1175±202.66)cpm/2000cells、(771±164.86)cpm/2000cells,明显低于对照组(1608±204.32)cpm/2000cells(均P<0.01);②10-5mol/LSim作用CFs6h、12h、24h、36h、48h,随着Sim作用时间的延长,CFs的3HTdR掺入率呈递减趋势,与时间呈明显的负相关(r=-919,P<0.01)。【结论】Sim可抑制CFsDNA的合成,即可抑制CFs增殖,且呈浓度时间依赖性。
[Objective]To investigate the effects of simvastatin on the DNA synthesis of rat cardiac fibroblasts (CFs).[Methods]CFs of neonatal Sprague-Dawley (SD) rats were isolated by trypsin digestion method and growth-arrested CFs were stimulated with varying concentrations and time of Sim. DNA synthesis was measured by 3H-Thymidine (3H-TdR) incorporation.[Results]The results showed that: ①Sim decreased 3H-thymidine incorporation in cardiac fibroblasts in a concentration dependent manner.3H-TdR incorporation values of 10 -6 mol/L sim and 10 -5 mol/L sim group(1175±202.66 cpm/2000 cells,771±164.86 cpm/2000 cells,respectively) were significantly lower than that of the control (1608±204.32 cpm/2000cells) (both P< 0.01 ); ②With the increase of time of the effects of 10 -5 mol/L Sim on CFs (6h, 12h, 24h, 36h, 48h), 3H-TdR incorporation of CFs decreased gradually, there was obviously negative relevance between 3H-TdR incorporation and time(r=-919,P<0.01).[Conclusion]The results indicate that Sim decreases DNA synthesis and CFs number.
出处
《医学临床研究》
CAS
2005年第7期886-888,共3页
Journal of Clinical Research
