摘要
目的体外研究以评价鲎抗内毒素因子模拟肽(modelingpeptidesfromthelimulusanti-lipopolysaccharidefactor,MPLALFs,Ms)中和内毒素(lipopolysaccharide/endotoxin)的活性。方法Ms结合内毒素的活性采用生物传感器技术测定,结合力以反应速率值(Kon)和Kd值表示;中和内毒素活性以鲎试验动态浊度法进行分析。结果生物传感器技术测定结果表明,M1、M2、M3、M4、M6、M8、M10、M0与LPS055∶B5的亲和力反应速率分别为(840±5·716)、(549±6·532)、(842±6·530)、(627±2·450)、(996±5·716)、(814±8·982)、(556±1·633)和(635±2·449)arcsecond(弧度/秒),以M4活性最强,M1活性最弱。M4与LPS的反应Kd值为72·377μmol/L。鲎试验结果与生物传感器测定结果基本一致。结论M4具有较强的体外中和内毒素活性。
Objective To evaluate the endotoxin-neutralizing activity of modeling peptides from the limulus antilipopolysaccharide factor (MPLALFs, Ms) in vitro. Methods The endotoxin-binding activity of Ms was examined by biosensor technique and shown in values of Kon and Kd. The endotoxin-neutralizing effect was analyzed by limulus amebocyte lysate test. Results The biosensor technique results showed that the Kon values of M_0, M_1, M_2, M_3, M_4, M_6, M_8 and M_ 10 binding to LPS 055∶B5 were (840±5.716), (549±6.532), (842±6.530), (627±2.450), (996±5.716), (814±8.982), (556±1.633) and (635±2.449) arc second, of which M_4 and M_1 had the highest and lowest endotoxin-binding activity, respectively. The M_4 reacted to LPS with a Kd of 72.377 μmol/L. The results obtained by the limulus amebocyte lysate test were the same with those from the biosensor technique. Conclusion M_4 has a potential good endotoxin-neutralizing effect in vitro.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2005年第13期1323-1325,共3页
Journal of Third Military Medical University
基金
重庆市科委攻关课题资助项目(2000)~~
关键词
鲎抗内毒素因子
模拟肽
内毒素
生物传感技术
鲎试验
limulus antilipopolysaccharide factor
modeling peptide
LPS
biosensor technique
limulus amebocyte lysate test
