摘要
目的探讨中药汤剂国圣一号(丹参、生首乌、当归、败酱草、莱菔子等)对博莱霉素(BLM)A5所致大鼠肺间质纤维化的干预作用及其机制。方法雄性Wistar大鼠48只,随机均分为空白对照组、BLM模型组、中药国圣一号治疗组、氢化考的松治疗组4组。大鼠气管内一次性注入BLM5mg/kg制成肺间质纤维化模型,模型制备后第2天,各组给予相应处理。4组动物分别于第14、28天各处死6只,提取肺组织,苏木精-伊红染色后进行病理半定量分析,并应用免疫组织化学法分析白介素(IL)-6和转化生长因子(TGF)-β1在肺组织的表达。采用分光光度法测定肺组织匀浆羟脯氨酸含量。结果应用BLMA5成功建立肺纤维化模型。中药国圣一号治疗组大鼠肺纤维化较BLM模型组明显改善,造模第28天TGF-β1明显下降,但对IL-6无抑制作用,总体疗效弱于氢化考的松治疗组。结论中药国圣一号具有显著抑制BLM致大鼠肺纤维化的作用,抑制TGF-β1的表达是其可能的机制之一。
Objective To investigate the effect and the mechanism of Chinese medicine GUOSHENG-YIHAO(consisting of Salvia Miltiorrhiza, Raw Polygonum Multiflorum, Angelica Root, Dahurian Patrinia Herb, Semen Raphani, etc.) in bleomycin-induced pulmonary fibrosis rats. Methods 48 male Wistar rats were divided into four groups randomly: normal control group, model group treated with bleomycinA5, GUOSHENG-YIHAO treated group, and hydrocortisone treated group. Each group included 12 rats. The rats were treated by single intratracheal instillation of sterile saline solution (the normal control group) or with 5 mg/kg body weight of bleomycin(the other groups). On the next day, each group was given corresponding drugs respectively. On days 14 and 28 after the bleomycin instillation, 6 rats of each group were killed for the lung tissue undertaken HE staining. Semi-quantitative analysis was used to measure the level of the lung fibrosis. The expression of transforming growth factor(TGF)-β1 and interleukin(IL)-6 in the lungs were determined by immunohistochemistry. Hydroxyproline was measured in lung tissue homogenates by spectrophotometer.Results The bleomycin-induced pulmonary fibrosis model was successfully constructed. In the rats of the herbal group, the pulmonary fibrosis was much improved as compared with that of the model group. TGF-β1 level in the herb group on the 28th day after the model creation decreased obviously, but no effect was seen on inhibition of IL-6. As a whole, the theraputic effect of GUOSHENG-YIHAO treated group was less than that of hydrocortisone treated group. Conclusions The GUOSHENG-YIHAO has a notable effect in bleomycin-induced pulmonary fibrosis rats, inhibition of TGF-β1 dexpression is one of its possible mechanisms.
出处
《上海医学》
CAS
CSCD
北大核心
2005年第6期499-503,F006,共6页
Shanghai Medical Journal
