摘要
目的:观察久泻宁颗粒对大鼠溃疡性结肠炎模型的影响,为临床用药的有效性提供实验依据。方法:采用异种异体结肠黏膜组织致敏法和三硝基苯磺酸(TNBS)和无水乙醇局部灌肠相结合。将SD大鼠随机分为空白对照组,模型对照组,阳性对照组,久泻宁颗粒高、中、低剂量组。连续给药20天,末次给药后24h,大鼠腹主动脉取血,采用ELISA法测定大鼠细胞因子IL-2、TNF-α以及免疫球蛋白IgG、IgM含量。结果:久泻宁颗粒中、高剂量组可升高IL-2,与UC模型组比较,差异非常显著(P<0.01);久泻宁颗粒各剂量组可降低TNF-α,与UC模型组比较,差异有显著意义(P<0.05～P<0.01)。久泻宁中剂量组可显著降低IgG水平,久泻宁中、高剂量组可显著降低IgM水平,与UC模型组比较,差异有显著意义(P<0.05)。结论:久泻宁颗粒对溃疡性结肠炎模型大鼠有显著的治疗作用。
Objective:To study the effect of Jiuxiening Granula on the rat model of ulcerative colitis.Methods:SD rats were randomly divided into six groups.Rats in positive control group were treated with Bupiyichang Wan and those in three treatment groups treated with low middle and high dosage of Jiuxiening Granula respectively for 20 days continuously.Ulcerative colitis models were established by immune method combined with local stimulation of TNBS.Twenty-four hours later,levels of IL-2,TNF-α and IgG,IgM were measured by ELISA.Results:Levels of IL-2 in middle and high dosage of Jiuxiening Granula increased significantly in comparison with that in model group(P<0.01);Levels of TNF-α in low,middle and high dosage of Jiuxiening Granula reduced significantly in comparison with that in model group(P<0.05 or <0.01).Levels of IgG in middle dosage of Jiuxiening Granula reduced significantly in comparison with that in model group(P<0.05).Levels of IgM in middle and high dosage of Jiuxiening Granula reduced significantly in comparison with that in model group(P<0.05).Conclusions:Jiuxiening Granula has significant treating effect on ulcerative colitis in the rat models.
出处
《中医药学刊》
2005年第7期1172-1174,共3页
Study Journal of Traditional Chinese Medicine
基金
福建省自然科学基金课题(C0210018)
