摘要
目的了解肺炎支原体(MP)对红霉素耐药情况及耐药机制。方法对60例临床证实为MP感染患儿鼻咽分泌物或咽拭子标本进行分离培养,药物敏感试验筛选耐药株,进行耐药基因检测。结果60例标本中,分离阳性株5例,耐药株4例,占80%。敏感株和标准株的23SrRNA基因序列与基因库的MP基因序列相同,4例耐药株的23SrRNA基因发生点突变,两株突变位点在2063,G替代了A,另两株突变位点在2064,G替代了A。5株分离株erm编码的甲基化酶基因均为阴性。结论MP对红霉素有耐药株产生,4株MP红霉素耐药株的耐药分子基础是23SrRNA基因发生点突变,未发现erm编码的甲基化酶基因。
Objective To study erythromycin resistance and determine molecular mechanisms in mycoplasma pneumoniae.Methods Sixty clinical samples were cultured, drug sensitive test was done to identify resistant strain, and then drug-resistant gene was screened.Results Five clinical strains were isolated from 60 samples. Among them four strains were resistant to erythromycin and the percentage was 80%. The 23 S rRNA gene sequences of the susceptible strain and the reference strain were identical to mycoplasma pneumoniae gene in genebank. While four resistant strains had a point mutation respectively, two had A to G transition at position 2063, the other two had A to G transition at position 2064 . None of the five strains had erm gene.Conclusions There is erythromycin resistance in mycoplasma pneumoniae and the molecular mechanism is point mutation in 23 S rRNA, erythromycin ribosomal methylase gene erm gene isn′t founded.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2005年第7期666-667,i004,共3页
Journal of Applied Clinical Pediatrics
基金
北京市中医管理局重点扶植项目资助(2001-002)
