摘要
用９９ｍＴｃ标记抗人活化血小板ＧＭＰ－１４０ＭＣＡｂＳＺ－５１，对实验性狗股动脉和股静脉血栓模型进行了免疫显像研究．结果表明：血栓显示清晰，其最佳显像时间为注射９９ｍＴｃ－ＳＺ－５１后４～６小时；９９ｍＴｃ－ＳＺ－５１引血液半清除时间Ｔ１／２α＝１６．２５±１３．３９分钟、Ｔ１／２β＝７．２８±１．１５小时；注射９９ｍＴｃ－ＳＺ－５１后８小时处死狗，离体血栓／血液和血栓／周围肌肉单位质量放射性比值：动脉血栓为５７．０９±１２．２４和１７７．４６±１１９．９３，静脉血栓为６．４３±４．７０和９４．４６±５７．５９；９９ｍＴｃ标记非特异ＩｇＧ，未见血栓显示．提示９９ｍＴｃ－ＳＺ－５１具有活体内导向定位血栓的能力，用于血栓性疾病的放免显像诊断有一定的可行性。
Abstract In experimental canine models of femoral arterial and venous thrombosis,the feasibility of thrombus imaging with 99mTc labeled monoclonal antibody SZ-51 to an α-granule membrane protein (GMP-140)on the surface of activated platelets was investigated.Both the avterial and venous thrombi were clearly discernible at 2-8h after injection. The optimal imaging time was 4-6h.These results were confirmed by in vivo images of thrombosed arteries and veins followed by killing the dogs at 8h post injection. Quantitative imaging analysis showed that the ratios between the thrombus and the opposite vessel were increased strikingly over time. The ratios of thrombus to blood or surrounding muscle were 57.09±12.24 and 177.46±9.00 for arterial thrombosis and 6.43±4.70 and 83.22±68.98for venous one at the time of sacrifice, respectively. Radiolabeled antibody was cleared from the blood with T1/2αof 16.25±13.39 min and T1/2βof 7.28±1 .15 h. These results show that it is feasible to noninvasively detect the thrombus formation in vivo using 99mTc labeled monoclonal antibody SZ-51.
出处
《中华核医学杂志》
CAS
CSCD
北大核心
1994年第1期35-37,T004,共4页
Chinese Journal of Nuclear Medicine
