摘要
通过给新生Ｗｉｓｔａｒ大鼠连续１０次（每日１次）１－甲基－３－硝基－１亚硝基肌（ＭＮＮＧ）灌胃，成功地诱发出腺胃癌、腺瘤和异型增生等病变。在０．４ｍｇ／只剂量组，这三种病变的诱发率分别为３９％、５０％和１００％，腺胃癌的诱发率与ＭＮＮＧ剂量成正比，雄性大鼠高于雌性（Ｐ＜０．０２），７０％病变发生在胃窦部。本模型具有致癌物用量小、易定量、给药期短、省时省力和不易污染环境等优点。研究还发现６个腺胃癌中有４个ＤＮＡ具有使ＮＩＨ／３Ｔ３发生恶性转化的作用，这种转化表现为对Ｂａｌｂ／ｃ裸鼠有致瘤性，提示在这种腺胃癌ＤＮＡ中存在转化基因。
denocarcinoma, adenoma and dysplasia of the stomach of adult Wistar rats were induced following administration of MNNG for 10 days by gavage when they were new一born. The incidence of the three types of pathological lesions at the dose of 0.4mg MNNG per rat being 39%,50%and 100%respectively.Induction of gast ric adenocarcinoma is dose一dependent. The incidence of adenocarcinoma in male rats being significantly higher then that of female rats(P<0.02). 23 of 33(70%)induced cancers were found in the gastric antrum. By the use of DNA cotransfection technique and the assay of carcinogenicity in the Balb/c nude mice, it was also found that the DNA of 4of the 6 induced gastric carcinomas could transform NIH /3T3 cells into malignant cells,an indication that the induced cancer DNA contains transforming gene.
出处
《中华病理学杂志》
CSCD
北大核心
1994年第5期293-295,T058,共3页
Chinese Journal of Pathology
基金
国家自然科学基金
关键词
疾病模型
腺癌
胃肿瘤
Disease models
animal Adenocarcinoma
