摘要
目的研究可溶性血管内皮生长因子受体1(solublefms-liketyrosinekinasereceptor1,sFlt-1)mRNA在子痫前期患者胎盘组织中的表达与定位,探讨其与子痫前期的关系。方法通过半定量RT-PCR及原位杂交方法分别检测子痫前期患者和健康孕妇胎盘组织中sFlt-1mRNA的表达及定位。结果正常胎盘组织和子痫前期胎盘组织中均存在sFlt-1mRNA,子痫前期胎盘组织中sFlt-1mRNA表达明显高于正常胎盘组织(P<0.01)。sFlt-1mRNA主要分布在胎盘绒毛滋养细胞及血管内皮细胞胞浆内。结论胎盘组织中sFlt-1的高表达可能参与了子痫前期的病理生理过程。
Objective:To investigatethe expression and location of soluble fms-like tyrosine kinase receptor 1 (sFlt-1) mRNA in placenta of preeclampsia and its role in preeclampisa pathophysiology. Methods: Semi-quantitative RT-PCR was carried out to detect the level of sFlt-1 mRNA in placental tissue of 10 preeclampsia (study group) and 10 normotensive pregnancies (control group); sFlt-1 mRNA was pinpointed by in situ hybridization. Results: The sFlt-1 mRNA was expressed in both preeclampsia and normal placenta. However, its levels were significantly higher in the former than in the latter (P<0.01); sFlt-1 mRNA was located in trophocyte of placenta by in situ hybridization. Conclusion: Higher expression of sFlt-1 may be associated with the pathogenesis of preeclampsia.
出处
《中国优生与遗传杂志》
2005年第6期19-21,18,共4页
Chinese Journal of Birth Health & Heredity
基金
国家自然科学基金资助项目(30070787)
