摘要
目的利用抗DR5单克隆抗体(mAb),检测死亡受体5(DeathRecepter5)在肿瘤细胞系表面的表达。方法sDR5免疫BALB/c小鼠,小鼠脾细胞与SP2/0-Ag14细胞在50%PEG条件下融合,获得能够分泌抗DR5单克隆抗体的杂交瘤细胞株,利用杂交瘤细胞株分泌的抗DR5特异性单克隆抗体,采用流式细胞仪技术测定抗DR5mAb结合至细胞表面的量来分析死亡受体在肿瘤细胞系的表达情况。结果不同肿瘤细胞表面DR5的表达水平分别为Jurkat细胞91.61%、NS-10.66%。结论抗DR5mAb能够特异性与DR5结合。DR5在Jurkat细胞系高水平表达,NS-1细胞系几乎不表达。该特异性mAb在研究TRAIL受体的生物功能上是一种有用的工具,对研究DR5在组织和细胞系表达十分有价值。
Objective: To establish hybridomas that produce anti-death receptor 5 mAbs and check surface expression of death receptor on cell lines.Methods: BALB/c mice were immunized with sDR5 . The spleen cells were fused with the myeloma SP2/0-Ag 14 in the presence of 50% polyethylene glycol to acquire hybridomas that produce anti-death receptor 5 mAbs. Surface expression of death receptor was determined by flow cytometric analysis by measuring the binding of anti-DR5 mAb.Results: The expression levels of the different tumor cell surface DR5 were respectively 91.61% Jurkat cells and 0.66% NS-1 cells.Conclusion: These results demonstrate that anti-DR5 mAb is able to specifically bind to DR5 and that DR5 is expressed at high levels on Jurkat cell lines. but NS-1 cells do not express membrane DR5.The DR5 specific mAb described here will serve as a valuable tool for studying the biology of TRAIL the receptors. The anti-DR5 mAb generated in the present study may be useful for investigating DR5 expression in tissues or cell lines.
出处
《河南大学学报（医学版）》
CAS
2005年第2期17-19,共3页
Journal of Henan University:Medical Science
基金
河南省杰出人才创新基金(0321001800)。
关键词
死亡受体5
肿瘤
表达
Death receptor 5
Tumor
Expression
