摘要
目的观察芪灵益肝煎、芪灵益肝煎CO2超临界萃取液、环磷酰胺(Cyc)的含药血清对小鼠腹水型肝癌细胞株HcaF25/CL16A3(H22)端粒酶反转录亚单位(TERT)表达的影响。方法利用血清药理学方法,以Cyc为阳性对照,用免疫组化技术观察小鼠腹水型肝癌细胞株H22TERT的表达。结果经大、小剂量芪灵益肝煎、芪灵益肝煎CO2超临界萃取液、Cyc的含药血清作用后小鼠腹水型肝癌细胞株H22TERT的表达均下调,与对照组比较,差异有明显的统计学意义(P<0.01),而下调TERT的程度在4组组间比较差异无统计学意义(P>0.05)。结论大、小剂量芪灵益肝煎、芪灵益肝煎CO2超临界萃取液、Cyc均可下调H22TERT的表达,而且下调TERT的程度相当。
AIM To observe the expression of telomerase reverse transcriptase (TERT)through immunohistochemical technique on hepatocellular carcinoma cell line H 22 after treated with the serum of these drugs: cyclophosphamide(Cyc),Qilingyiganjian(QLYGJ)and its liquid of CO2 supercritical fluid extraction(SFE). METHODS With the method of seropharmacology,the expression of TERT by immunohistochemical technique was studied by contrast of Cyc,that was,positive control. RESULTS The group of Cyc,high and low dose QLYGJ,and its liquid of CO2 SFE had positive expression.Rank sum test showed that the expression of TERT was downsized,and there was apparent deference from that of control group(P<0.01).While among the 4 groups of serum treated with drugs, there were no statistical differences(P>0.05). CONCLUSION All of there drugs:Cyc,big and small dose QLYGJ,the liquid of CO2-SFE can downregulate the expression of TERT,furthermore,the degree of their downregulating TERT makes no statistical difference.
出处
《中国临床药学杂志》
CAS
2005年第3期180-182,共3页
Chinese Journal of Clinical Pharmacy
关键词
肝癌
端粒酶
CO2超临界萃取
telomerase
hepatic carcinoma
CO2 supercritical fluid extraction
