摘要
目的 研究蛋白酶 -抗蛋白酶失衡在兔创伤性肺挫伤中的作用。方法 家兔随机分为 2组 ,创伤组 (n =8)和对照组 (n =8) ,采用兔单侧肺挫伤生物撞击模型 ,动态观察血浆中性粒细胞弹性蛋白酶(NE)、α1 抗胰蛋白酶 (α1 AT)、肿瘤坏死因子 α(TNF α)及白介素 8(IL 8)含量变化 ;活杀动物后取肺组织比较湿 /干重比值 (W/D)、丙二醛 (MDA)、髓过氧化物酶 (MPO)的变化 ,观察支气管肺泡灌洗液 (BALF)中上述细胞因子和炎症介质的变化 ,进行肺组织的病理学光镜检查。结果 创伤组与对照组相比较 ,血浆和BALF中NE的活性升高、血浆α1 AT含量先降低后升高、TNF α、IL 8含量均明显升高 (P <0 .0 1或P <0 .0 5 ) ,兔肺组织损伤以肺出血、水肿和中性粒细胞 (PMN)浸润为主要表现。结论 肺挫伤后 ,PMN激活并释放NE ,使蛋白酶 -抗蛋白酶失衡 ,在创伤性急性肺损伤中有重要的作用。
Objective To investigate the role of protease-antiprotease imbalance in pulmonary contusion after the chest impact trauma in the rabbits.Methods Rabbits were randomly divided into two groups: traumatic group (n=10) and the normal control group (infusion of saline,n=8).The isolated lung contusion model of the rabbit was established,induced by a bioimpact machine.The plasma concentrations of elastase and α 1 -antitrypsin (α 1 -AT) were determined.The changes in tumor necrosis factor-A(TNF-α) and interleukin-8(IL-8) contents were measured both in plasma and bronchalvelor lavage fluid (BALF).The contents of malondialdehyde (MDA) and myeloperoxidase (MPO) were detected in the lung tissue.The wet/dry weight ratio,the contents albumin in BALF and the histological features of lung were examined.Results Compared with the control group,the activity of NE,TNF-α and IL-8 was increased both in plasma and BALF,but the concentrations of α 1 -AT were reduced initially,and then elevated later in plasm.The contents of MDA and MPO were raised in the lung tissue.The ratio of W/D and albumin in BALF were higher than that of the control group(P<0.01 or P<0.05).Pathological examination revealed marked changes,including alveolar hemorrhage, pulmonary edema,leukocyte extravasation and infiltration.Conclusion The imbalance of protease-antiprotease might play an important role in the pathogenesis of traumatic pulmonary contusion.
出处
《创伤外科杂志》
2005年第2期92-95,共4页
Journal of Traumatic Surgery
