摘要
目的研究大鼠桡神经钳夹伤后背根神经节(DRG)和脊髓nNOS免疫阳性神经元的变化,探讨NO是否参与大鼠桡神经钳夹伤后的DRG和脊髓水平的痛觉调制。方法用辣根过氧化物酶追踪大鼠桡神经的由来;大鼠桡神经钳夹伤结合免疫组化法,研究桡神经钳夹伤后DRG鄄和脊髓的nNOS免疫阳性结构变化。结果(1)大鼠桡神经的组成范围在C5～T1;(2)大鼠桡神经钳夹伤后,DRG内nNOS免疫阳性神经元的变化难以分析:脊髓后角nNOS免疫阳性结构数量减少、免疫强度下降。结论大鼠桡神经钳夹伤后,脊髓nNOS免疫阳性结构发生可塑性变化,NO在桡神经钳夹伤后的痛觉调制中有一定的作用。
Objective By studying the changes of nNOS immuno-positive neu ron numbers of rat's spinal ganglion and spinal lord following radial nerve clam ping injury, to explore the possible involvement of NO in pain modulation on the DRG and spinal cord. Methods The radial nerve was traced with a classic HRP hi stochemistrical approach. On a radial nerve injury model by mechanical clamping, the nNOS immuno-positive neurons were observed after immuhistochemistry of anti body against nNOS with a two-step methods. Results The radial nerve was compose d with the fibers of the spinal nerve roots from C5 to T1 In the radial nerve in jury model, changes of the nNOS immuno-positive neurons were hard to analysis. B ut there was obvious decline either in the number or in the intensity of such ki nd of immno-positive neurons. Conclusion There are obvious changes of the archi tecture plasticity of the nNOS immuno-positive neurons. NO may be an important m odulate of pain on the DRG and spinal cord level.
出处
《解剖学研究》
CAS
2005年第2期110-112,F004,共4页
Anatomy Research
关键词
桡神经
背根神经节
脊髓
一氧化氮合酶
Radial nerve
Spinal cord
Dorsal root ganglion
Nitric ox ide synthecse
