摘要
目的通过敏感的荧光方法,定量分析慢性低灌注性脑缺血再灌注后大鼠血脑屏障(BBB)的通透性。方法雄性Wistar大鼠35只。大鼠分为模型再灌注组(n=25),模型不灌注组(n=5)和对照组(n=5)。模型再灌注组和模型不灌注组行右侧颈总动脉和颈外静脉端端吻合,同时结扎上矢状窦,建立大鼠脑动静脉瘘的动物模型;对照组单纯行右侧颈总动脉结扎。2周后,每组大鼠实验结束前2h尾静脉注射伊文思蓝(EB)。模型再灌注组阻断右侧颈总动脉和颈外静脉吻合部位形成缺血再灌注,采用EB荧光定量的方法分别观察再灌注2、3、9、24、48h时BBB的通透性。结果再灌注2h时,脑组织EB的含量为(0.935±0.166)μg/g,与对照组(0.489±0.132)μg/g相比显著增高(P<0.05),与模型不灌注组(0.713±1.217)μg/g相比显著增高(P<0.05)。再灌注24h达高峰,为(5.231±1.183)μg/g,再灌注48h趋于平稳,为(5.522±1.124)μg/g。结论慢性低灌注性脑缺血BBB通透性2周后增高;再灌注后2hBBB的通透性增高更具明显,再灌注24h达高峰,再灌注48h趋于稳定。
Objective To quantitatively analyze the permeability of blood-brain barrier with the hypersensitive fluorescent methods during reperfusion after chronic hypoperfusion of cerebral ischemia.Methods The male Wistar rats (n= 35) were divided into 3 groups:reperfusion group (n= 25),no reperfusion group (n= 5) and control group (n=5).The end-to-end anastomosis of the right common carotid artery and the right external jugular vein was performed,the superior sagittal sinus was ligated and the the rat model of carotid-jugular arteriovenous fistula was established in the reperfusion group and the no reperfusion group;Only the right common carotid artery was ligated in control group.Two weeks later,the Evansblue was injected into the caudal vein at 2 h before the end of experiment in every group.The anastomosis locus was blocked to form the reperfusion after the ischemia in the reperfusion group.The permeability of blood-brain barrier was observed by the EB fluorescence quantitative method at 2 h,3 h,9 h,24 h and 48 h after the reperfusion.Results Two h after the reperfusion,the content of EB in the cerebral tissue was (0.935± 0.166) μg/g,which was obviously increased (P< 0.05) as compared with the control group (0.489± 0.132) μg/g and the no reperfusion group (0.713± 1.217)μg/g.It peaked at 24 h (5.231± 1.183) μg/g and then tended to stable at 48 h (5.522± 1.124) μg/g after the reperfusion.[WT5”HZ]Conclusion The permeability of blood-brain barrier was increased at 2 weeks after the model established,further increased at 2 h after the reperfusion,peaked at 24 h and tended to stable at 48 h after the reperfusion.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第7期858-859,共2页
Chinese Journal of Experimental Surgery
基金
全军十五计划青年基金资助项目(01Q001)
