摘要
将固定化鸡肝酯酶酶柱应用于流动注射分析系统中,以峰值时间和峰高为指标,考察了底物浓度、缓冲液流量和酶柱长度对流出曲线的影响.结果发现,当底物进样量体积一定时,底物浓度对峰值时间无显著影响,而对峰高的影响近似符合酶催化反应中的米氏方程;在现有酶活力水平条件下,底物浓度应选择为160mmol/L;缓冲液流量在0.38～0.75mL/min范围内,峰值时间与流量呈反比,即每提高1mL/min,峰值时间约缩短13.3min,而流量对峰高的影响呈不对称的下凹曲线关系;峰值时间和峰高分别随酶柱长度的延长而延迟或升高.
Immobilized chicken liver esterase was used in a lab FIA system. With curve peak time and peak height as criteria, the effects of substrate concentration, flow rate of buffer liquid and length of enzyme column have been studied. It was found that concentration of substrate has no effect on peak time and the curve peak height against substrate concentration releases a model similar to Michaelis equation with fixed injection volume of substrate. Under existing activity of immobilized enzyme, 160 mmol/L can be chosen as concentration of substrate. When buffer flow rate is controlled from 0.38 mL/min to 0.75 mL/min, peak time of the curves has a negative linear response to buffer flow rate and the proportional coefficient is 13.3 min/(mL·min-1). However peak height varies with buffer flow rate in concave form. Both peak time and peak height increase with the increase of length of enzyme column.
出处
《上海理工大学学报》
EI
CAS
北大核心
2005年第3期189-193,共5页
Journal of University of Shanghai For Science and Technology
基金
国家自然科学基金资助项目(50176032)
上海市科委启明星计划资助项目(02QF14030)
上海市教委青年基金资助项目(02GQ21).
关键词
流动注射分析
酶柱
流出曲线
flow injection analysis
enzyme column
flow out curve
