摘要
背景:记忆力下降是放射性脑病早期的主要临床表现,已有许多人认为神经节苷酯在神经修复中对记忆功能具有改善作用。目的:研究神经节苷酯对鼠脑放射性损伤后空间学习记忆能力减退的影响。设计:以动物为观察对象的随机对照实验。单位:中山大学第二附属医院的神经科和放射科。材料:实验于2001-03/2002-05在中山大学附属第二医院实验室完成。选取SD大鼠80只,分为空白对照组、神经节苷酯治疗组、生理盐水治疗组、未干预组,20只/组。干预:神经节苷酯治疗组、生理盐水治疗组、未干预组大鼠麻醉后头部接受60Coγ射线照射,7Gy/次,1次/d,连续照射6d,总剂量42Gy。空白对照组麻醉后不予照射。接受照射的3组每天照射后隔1h给药,神经节苷酯治疗组腹腔注射神经节苷酯30mg/kg;生理盐水治疗组注射等量的生理盐水,1次/d,连续6d;空白对照组和未干预组不给药。评估:①照射结束后采用Morris水迷宫的定位航行实验,通过记录大鼠寻找平台所需时间(潜伏期)来测定大鼠对水迷宫的学习记忆能力。②采用空间搜索实验,通过记录大鼠在120s内搜索平台的路线图,测量其平台象限的游泳距离占总距离的百分比,从而测定大鼠学会寻找平台后,对平台空间位置记忆的能力。③迷宫试验结束后将神经节苷酯治疗组、生理盐水治疗组、未干预组大鼠断头取?
BACKGROUND:Memory loss is the main presentation during the earlier stage of ra diative encephalopathy,and it was reported that ganglioside(GM1) played importan t role in neural rehabilitation,particular in the improvement of memory. OBJECTIVE:To study the improving effect of GM1 on spatial learning and memory retardation in rats following radiative encepholopathy. DESIGN:Randomized control and comparative observing study based on the experim ental animals. SETTING:Department of Neurology and Department of Radiation of Second Affiliat ed Hospital of Sun Yat sen University. MATERIALS:This study was carried out at the Laboratory of the Second Hospital Affiliated to Sun Yat sen University between March 2001 and May 2002.Totally 80 SD rats were randomly selected and divided into control group,GM1 treatment gro up,physiological saline group and non intervention group with 20 rats in each g roup. INTERVENTIONS:Rats in GM1 group,physiological saline group and non interventi on group subjected to head 60Coγirradiation of 7Gy each time after anesthesia,o nce a day for consecutive 6 days,and the total dosage was 42Gy while rats in con trol group did not receive irradiation after anesthesia.Rats in GM1 and physiolo gical saline(PS) group were given intraperitoneal injection of GM1 and physiolog ical saline of 30 mg/kg respectively at 1 hour after each time of radiation,once a day for consecutive 6 days but not in control group and non intervention gro up.Evaluation:①After irradiation,morris water labyrinth navigation test was us ed to assess the capability of learning and memory of rats by the time for reach ing platform(latency);②Spatial searching test was used to detect their spatial memory after learning how to reach the platform by recording the way of rats sea rching the platform in 120 s and calculating the percentage of swimming distance in platform quadrant in the total distance;③After labyrinth test,brains were t aken out of the rats in GM1 group,PS group and non intervention group for obser ving the histolog
出处
《中国临床康复》
CSCD
北大核心
2005年第16期254-256,共3页
Chinese Journal of Clinical Rehabilitation
基金
广东省卫生厅科研基金资助项目(B2004048)~~
